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LV Centramate™ Lab Tangential Flow Filtration System

Designed for Maximum Product Recovery for
Lab-Scale or Scale-Up Process Volumes up to 4 L

  • Scale-up/scale-down device for development of tangential flow filtration (TFF) processes, biopharmaceutical small batch production, scale-down investigations, and production of materials for safety and efficacy studies (Phase I clinicals).
  • Identical path lengths provide linear scale-up to Pall Centramate and Centrasette™ cassette holders. The LV Centramate holder may be attached to other laboratory TFF systems to improve their scalability characteristics.
  • Low hold-up volume allows high concentration factors to be achieved from small starting volumes. A complete TFF system scalable to full production plant can be optimized using the volumes typically generated in the development or discovery lab.
  • Easy connections through luer lock fitted ports with polished 316L stainless steel to ensure the same compatibility characteristics as production-scale Centrasette holders.
  • All product-wetted parts comply with pharmaceutical standards.

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."
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Description

Designed for Maximum Product Recovery for
Lab-Scale or Scale-Up Process Volumes up to 4 L

  • Scale-up/scale-down device for development of tangential flow filtration (TFF) processes, biopharmaceutical small batch production, scale-down investigations, and production of materials for safety and efficacy studies (Phase I clinicals).
  • Identical path lengths provide linear scale-up to Pall Centramate and Centrasette™ cassette holders. The LV Centramate holder may be attached to other laboratory TFF systems to improve their scalability characteristics.
  • Low hold-up volume allows high concentration factors to be achieved from small starting volumes. A complete TFF system scalable to full production plant can be optimized using the volumes typically generated in the development or discovery lab.
  • Easy connections through luer lock fitted ports with polished 316L stainless steel to ensure the same compatibility characteristics as production-scale Centrasette holders.
  • All product-wetted parts comply with pharmaceutical standards.

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."

Specifications

Materials of Construction

  • Membrane Cassette Filter Media: Supor® [hydrophilic polyethersulfone (PES)], and Omega™ (low protein-binding, modified PES) membranes
  • Cassette Hardware
    • Cassette Holder Top and Bottom Plates: Type 316L stainless steel
    • Tie Rods and Washers: Type 304 stainless steel
    • Nuts: Bronze

Dimensions

  • Length: 22.8 cm (9 in.) Width: 8.3 cm (3.3 in.)

Typical Starting Volume

  • 0.02 m2 (0.2 ft2) cassette: 60 - 4,000 mL

Typical Hold-Up Volume

  • < 1 mL

Maximum Operating Temperature

  • 121 °C (250 °F)

Maximum Operating Pressure

  • 5 bar (500 kPa, 75 psi)

Typical Cross Flow Rate Per 0.02 m2 (0.2 ft2) Cassette (L/min)

  • Suspended Screen 0.2 - 0.4
  • Screen 0.12 - 0.16

Screen Selection Guideline

  • Suspended screen for light-tomoderate particle load solutions
  • Screen for optimized processing of particle-free protein solutions to high concentrations

Applications

  • Concentration of biomolecules
  • Recovery and purification of biomolecules from cell culture supernatants and lysates
  • Diafiltration, desalting, and buffer exchange of biomolecules
  • Fractionation of mixed molecule solutions

Type

Systems

FAQs

1 Q. How many times can a Minimate™ capsules be reused?
A. There are no specific guidelines for the total number of times a Minimate capsule can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Minimate capsules prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of capsules should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Minimate capsule drops below 50% of the original NWP value, it is suggested that a new capsule is used.

2 Q. How many times can a Centramate™ cassettes be reused?
A. There are no specific guidelines for the total number of times a Centramate cassette can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Centramate cassettes prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of cassettes should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Centramate cassette drops below 50% of the original NWP value, it is suggested that a new cassette is used.

3 Q. When should I order Centramate™ vs Centramate PE?
A. Certain applications require a fluid path that is free from metal. Our standard Centramate has stainless steel fittings and a 100% stainless steel cassette holder, so it would not be a good option for these applications. Pall’s Centramate PE has plastic fittings and the fluid path of the holder is polyethylene plastic. The pressure gauge that comes standard with the Centramate PE will have a stainless steel pathway, but the ¼” NPT fittings the system uses makes it easy to use a pressure gauge whose wettable parts will be free from metal.

4 Q. What do I need to attach an LV Centramate™ to the Minimate™ EVO system?
A. Pall recommends ordering the Minimate EVO Fittings kit, PN 97014, to get the additional 3-way stop cocks, hose barb adapters, and tubing needed to properly connect the LV Centramate holder to the Minimate EVO system. Contact your Pall representative or technical support if you have further questions.

5 Q. How many cassettes can I stack in a Centramate™ or Centramate PE?
A. You can stack up to 5 0.1 m2 T12 cassettes on Centramate, giving you up to 0.5m2 of effective filtration area. For the Centramate PE, only 4 0.1 m2 T12 cassettes will fit because of the thicker PE base. Stacking multiple cassettes in the holders increases the surface area of the membrane which gives a higher permeate flow rate and increases the throughput.

6 Q. Guidance on average recirculation flow rates and permeate flux rates for the Minimate™, LV Centramate™, Centramate PE/SS.
A. For the Minimate we recommend a recirculation flow rate of 30-40 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 4 ml/min. For the LV Centramate we recommend a recirculation flow rate of 120-160 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 15 ml/min. For the Centramate and Centramate PE we recommend a recirculation flow rate of 600-800 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 75 ml/min. Note: These are typical values. Actual values may be higher or lower depending on the membr

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