Vivid™ Lateral Flow Nitrocellulose membranes product photo  L

Vivid™ Lateral Flow Nitrocellulose membranes

With quality assured manufacturing and defined lot-to-lot consistency, our Vivid Lateral Flow Nitrocellulose offers a reliable media for development, manufacturing and implementation of lateral flow diagnostic point of care tests.

Membrane consistency for assay reproducibility and sensitivity

  • Increased sensitivity

    Vivid Lateral Flow Nitrocellulose (LFNC) membranes provide high sensitivity with dilute concentrations of target analytes.

  • Strong consistency and high duplication rate

    When measuring intra- and inter-lot performance for wicking time and thickness Vivid LFNC membranes provide consistent results, resulting in low defined coefficients of variation.

  • Consistent reproducibility

    Provides protein binding levels which are highly consistent to facilitate reliable assay performance.

  • Clear results

    Demonstrates low background levels which enable crisp capture lines and easy-to-read results.

  • Dependable performance

    Constructed using pure nitrocellulose that does not contain interfering substances or post-treatments that affect assay performance.

  • Clear surface appearance

    Controlled surface quality of the membrane ensures freedom from visual defects, discolouration and dust.

  • Result timing

    Quality assured wicking speed gives predictable result time.


Nitrocellulose membranes are the heart of lateral flow assays and require highly consistent wicking rate and thickness. Variations in either can have detrimental effects on assay sensitivity, reproducibility, and reagent consumption.

Vivid LFNC membranes are manufactured to ensure that these key parameters are tightly controlled with coefficients of variation (CV) between 5 and 10%.

Nitrocellulose is the globally preferred membrane substrate in diagnostic tests where antigenantibody binding occurs, e.g., pregnancy tests, urine-albumin tests and C-reactive protein (CRP) detection. Pall’s Vivid LFNC membranes fulfil all the criteria to enable the development and manufacture of a diverse range of tests with reliability and reproducibility, available with the following benefits:

  • Quality: designed and tested specifically for diagnosticapplications to ensure the materials meet stringent requirements for diagnostic assay development and manufacturing
  • Reliable, large-scale manufacturing capacity with lot to lot traceability
  • Reproducibility: defined coefficients of variation for key performance parameters result in consistent performance
  • Choice of defined capillary speeds to select sensitivity of diagnostic test
  • Straight and uniform migration front


Need Help?
Ask a Question
* Required

Description

With quality assured manufacturing and defined lot-to-lot consistency, our Vivid Lateral Flow Nitrocellulose offers a reliable media for development, manufacturing and implementation of lateral flow diagnostic point of care tests.

Membrane consistency for assay reproducibility and sensitivity

  • Increased sensitivity

    Vivid Lateral Flow Nitrocellulose (LFNC) membranes provide high sensitivity with dilute concentrations of target analytes.

  • Strong consistency and high duplication rate

    When measuring intra- and inter-lot performance for wicking time and thickness Vivid LFNC membranes provide consistent results, resulting in low defined coefficients of variation.

  • Consistent reproducibility

    Provides protein binding levels which are highly consistent to facilitate reliable assay performance.

  • Clear results

    Demonstrates low background levels which enable crisp capture lines and easy-to-read results.

  • Dependable performance

    Constructed using pure nitrocellulose that does not contain interfering substances or post-treatments that affect assay performance.

  • Clear surface appearance

    Controlled surface quality of the membrane ensures freedom from visual defects, discolouration and dust.

  • Result timing

    Quality assured wicking speed gives predictable result time.


Nitrocellulose membranes are the heart of lateral flow assays and require highly consistent wicking rate and thickness. Variations in either can have detrimental effects on assay sensitivity, reproducibility, and reagent consumption.

Vivid LFNC membranes are manufactured to ensure that these key parameters are tightly controlled with coefficients of variation (CV) between 5 and 10%.

Nitrocellulose is the globally preferred membrane substrate in diagnostic tests where antigenantibody binding occurs, e.g., pregnancy tests, urine-albumin tests and C-reactive protein (CRP) detection. Pall’s Vivid LFNC membranes fulfil all the criteria to enable the development and manufacture of a diverse range of tests with reliability and reproducibility, available with the following benefits:

  • Quality: designed and tested specifically for diagnosticapplications to ensure the materials meet stringent requirements for diagnostic assay development and manufacturing
  • Reliable, large-scale manufacturing capacity with lot to lot traceability
  • Reproducibility: defined coefficients of variation for key performance parameters result in consistent performance
  • Choice of defined capillary speeds to select sensitivity of diagnostic test
  • Straight and uniform migration front

Specifications

Membrane Thickness Wicking Rate (s / 4 cm)
Vivid 90
LFNC
190 – 230 μm includes 95 – 105 μm polyester support 70 - 110
Vivid 120
LFNC
190 - 230 μm includes 95 – 105 μm polyester support 95 - 135

Thickness

The thickness of the reaction membrane has a direct impact on its signal generation. Consequently, any variations both intra- or inter-lot will have an impact on Point of Care Test (POCT) device manufacturing and the final assay performance.

In the data drawn from 3 lots, measuring the thickness of the membrane every 2.5 cm down a sample piece of material, the coefficient of variation was measured as ≤4.6% for Vivid 120 LFNC membrane with average thickness for all sample points being 196.7 ± 8.1 μm (Graph 1) and for Vivid 90 LFNC membrane the coefficient of variation was ≤2.6% with average thickness for all sample points being 217.1 ±




Capillary Speed/Wicking

Assay sensitivity is maintained with consistency of the wicking rate of the nitrocellulose membrane. By maintaining a uniform sample front that travels along the membrane at a consistent speed, the target analyte is maintained in a homogenous concentration. This ensures that the target analyte present in the sample has adequate time to bind to the capture antibody resulting in reproducible results from lot to lot. The wicking rate of the Vivid 90 and Vivid 120 LFNC membranes both exhibit CV’s for intra- and inter-lot consistency of ≤ 10% (Table 2 and 3).

Membrane Inter-lot Wicking Rate (s / 4 cm) Average (s / 4 cm) Standard Deviation Coefficient of Variation
Lot 1 Lot 2 Lot 3
Vivid 90 LFNC 83.7 79.3 87.3 83.4 6.1 7.3%
Vivid 120 LFNC 130.7 117.3 117.7 121.9 7.8 6.4%
Table 2: Summary data of intrer-lot reproducibility (from three sample points) of capillary speed and inter-lot reproducibility across three lots.

Membrane Intra-lot Wicking Rate (s / 4 cm) Position on Sample Sheet Average (s / 4 cm) Standard Deviation Coefficient of Variation
Left Centre Right
Vivid 90 LFNC 80.7 84.3 85.3 83.4 2.5 2.9%
Vivid 120 LFNC 117.7 123.0 125.0 121.9 3.8 3.1%
Table 3: Summary data of intra-lot reproducibility (from three sample points) of capillary speed and inter-lot reproducibility across three lots.

Scanning Electron Microscopy

Type

Accessories and Spare Parts

Ordering Information

Description Wicking Rate (s / 4 cm) Width Length Pkg Part Number
Vivid 120 LFNC Sample 95 - 135 25 mm 300 mm 1 Strip VIV120SAMP
Vivid 120 LFNC 95 - 135 25 mm 3 metres 1 Roll VIV1202503R
Vivid 120 LFNC 95 - 135 25 mm 50 metres 1 Roll VIV1202550R
Vivid 90 LFNC Sample 70 - 110 25 mm 300 mm 1 Strip VIV90SAMP
Vivid 90 LFNC 70 - 110 25 mm 3 metres 1 Roll VIV902503R
Vivid 90 LFNC 70 - 110 25 mm 50 metres 1 Roll VIV902550R

Reviews

We appreciate your review of this product. Please login to your account to leave a review.