Centrifugal Filters

Pall’s centrifugal filter devices can accelerate sample processing, maximize sample recovery, add versatility and prevent solution bypass.

Accelerate sample processing – Concentrate and purify samples with starting volumes of < 50 µL to 60 mL.

Maximize sample recovery – Obtain high flow rates and low non-specific protein and nucleic acid binding.

Add versatility – Available in various membrane types; Nanosep®, Microsep®, Macrosep™, and Jumbosep™ devices are available with Omega™ (modified polyethersulfone) ultrafiltration membrane in a variety of MWCOs. Nanosep MF devices are available with low-binding Bio-Inert® (modified nylon) and wwPTFE (hydrophilic polytetrafluoroethylene) membranes. Microsep and Macrosep devices also are available with Supor® (polyethersulfone) membrane.

Prevent solution bypass – Membrane seals stop solution leakage, minimizing sample loss.

Easy visual identification – Devices are color-coded for a wide variety of membranes, ranging from 1k to 0.45 µm

Documents

Data Sheets

Info Sheet: Centrifugal Devices

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Pall Nucleic Acid Binding Nanosep® Centrifugal Device

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Specifications

Centrifugal Filter	Attributes	Values
Nanosep® Centrifugal Filters	Materials of construction	Sample Reservoir, Membrane Support Base and Filtrate Receiver: Polypropylene Filter Media: Omega (modified polyethersulfone) ultrafiltration membrane Nanosep MF Devices Sample Reservoir, Membrane Support Base and Filtrate Receiver: Polypropylene Filter Media: wwPTFE (hydrophilic Polytetrafluoroethylene) and Bio-Inert (modified nylon) membranes
	Effective filtration area	0.28 cm²
	Dimensions	Length (full assembly with cap): 4.5 cm (1.8 in.)
	Operating temperature range	0 – 40 °C (32 – 104 °F)
	Capacities	Maximum Sample Volume: 500 μL Final Concentrate Volume: 15 μL Filtrate Receiver Volume: 500 μL Hold-up Volume (membrane/support): < 5 μL
	pH range	1 – 14
	Maximum centrifugal force	14,000 x g
	Centrifuge	Fits centrifuges that accept 1.5 mL tubes
	Sanitization	Non-sterile. May be sanitized by filtering 70% ethanol through the device before use.
Microsep™ Centrifugal Filters	Materials of construction	Sample Reservoir, Membrane Support Base and Filtrate Receiver: Polypropylene Filter Media: Supor (polyethersulfone) and Omega (modified polyethersulfone) membranes Paddle: Polyethylene
	Effective filtration area	3.3 cm²
	Dimensions	Length: 12.0 cm (4.9 in.) Diameter: 17 mm (0.7 in.)
	Operating temperature range	0 – 40 °C (32 – 104 °F)
	Capacities	Maximum Sample Volume: 5.0 mL Final Concentrate Volume: 65 μL (swinging bucket) 100 μL (35o angle rotor) 80 μL (45o angle rotor) Filtrate Receiver Volume: 6.5 mL Hold-up Volume: 40 μL (membrane and paddle)
	pH range	1 – 14
	Maximum centrifugal force	7,500 x g (for ultrafiltration)
	Centrifuge	Fits rotors that work with standard 15 mL conical tubes and are capable of 3,000 to 14,000 x g
	Sanitization	Non-sterile. May be sanitized by filtering 70% ethanol through the device before use.
Macrosep® Centrifugal Filters	Materials of construction	Sample Reservoir, Membrane Support Base and Filtrate Receiver: Polypropylene Filter Media: Supor (polyethersulfone) and Omega (modified polyethersulfone) membranes Paddle: Polyethylene
	Effective filtration area	7.2 cm²
	Dimensions	Length: 12.0 cm (4.7 in.) Diameter: 50 mm (1.9 in.)
	Operating temperature range	0 – 40 °C (32 – 104 °F)
	Capacities	Maximum Sample Volume: 20 mL Final Concentrate Volume: As low as 450 μL, depending on rotor used Filtrate Receiver Volume: 22 mL Hold-up Volume: 80 μL (membrane and paddle)
	pH range	1 – 14
	Maximum centrifugal force	5,000 x g (for ultrafiltration)
	Centrifuge	Fits rotors that accept standard 50 mL conical end tubes
	Sanitization	Non-sterile. May be sanitized by filtering 70% ethanol through the device before use.
Jumbosep™ Centrifugal Filters	Materials of construction	Sample Reservoir and Filtrate Receiver: Polysulfone Sample Reservoir Cap: Polyethylene Filter Media: Omega (modified polyethersulfone) membrane Insert Without Membrane: High-density polyethylene Filtrate Receiver Cap and Insert Release: Polypropylene
	Effective filtration area	15.2 cm²
	Dimensions	Maximum Outside Diameter: 6 cm (2.4 in.) Height (Full Assembly with Cap): 11.3 cm (4.5 in.)
	Operating temperature range	0 – 40 °C (32 – 104 °F)
	Capacities	Maximum Sample Volume: 60 mL Final Concentrate Volume: 3.5 – 4 mL Filtrate Receiver Volume (Maximum): 60 mL Hold-up Volume: 0.2 mL (membrane/support)
	pH range	1 – 14
	Maximum centrifugal force	3,000 x g
	Centrifuge	Fits swinging bucket rotors that accept flat-bottomed 250 mL bottles and are capable of 1,000 to 3,000 x g
	Sanitization	Non-sterile. May be sanitized by filtering 70% ethanol through the device before use. The filtrate receiver and sample reservoir can be autoclaved. Do not autoclave the filter media.

Applications

Pall’s centrifugal filter devices can replace traditional separation techniques, such as column chromatography, preparative electrophoresis, alcohol or salt precipitation, dialysis, and gradient centrifugation, when performing the following: Protein or nucleic acid concentration Desalting Buffer exchange Deproteination of biological samples Fractionation of protein mixtures Separation of primers from PCR products Separation of labeled nucleic acids or proteins from unincorporated nucleotides Virus concentration or removal Clarification of cell lysates and tissue homogenates

Centrifugal Filters