Unique bi-axial agitation improves cell culture performance
The Allegro XRS 25 bioreactor system is a single-use bioreactor system with unique agitation and control properties designed for the cultivation of mammalian cells in suspension culture under controlled conditions. It is suitable for applications ranging from general life sciences research to seed train operations and full cGMP production at the 2 to 25 liter scale.
Because of its unique bi-axial agitation and the subsequent shorter mixing times and higher mass transfer properties, the Allegro XRS 25 bioreactor system produces superior performance compared to competitor rocker systems, as measured by higher density, better cell viability, and higher expression levels.
The Allegro XRS 25 bioreactor system rocks on two axes simultaneously (bi-axial agitation), producing a low turbulence, swirling pattern within the 3-dimensional XRS 25 biocontainer. Under similar agitation conditions, the Allegro XRS 25 bioreactor system mixes almost 3 times faster than conventional rockers (Table 1), and demonstrates much higher mass transfer properties as measured by kLa values (Table 2).
Summary of best mixing times observed in pH equilibration studies, 20 liter working volumes with conventional pH probes¹
|Conventional rocker||Maximum speed and rock angle: 42 rpm, 10.4 °||50 to 98 seconds2|
|Allegro XRS 25 bioreactor system||75% maximum speed, maximum long axis angle, 1/3 short axis angle: 30 rpm, 15 ° long, 5 ° short||16 seconds|
1 Mixing trial results from three repetitions for each device.
2 Times significantly different for edge/center measurements.
3 Mass transfer results from three repetitions for each device.
For additional details on mixing and mass transfer studies, see Pall application note USD 2899: Performance Mapping in the Pall XRS 20 Bioreactor System.
Summary of mass transfer coefficients observed in 20 liter working volumes as measured with nitrogen purge and recovery3
|System||Condition||kLa per Hour|
|Conventional rocker||Maximum speed and rock angle: 42 rpm, 10.4 °||42|
|Allegro XRS 25||40 rpm, 15 ° long, 15 ° short||73|
System Overview, Features and Benefits
Allegro XRS 25 bioreactor system – featuring the agitation platform, the single-use biocontainer and the mPath™ control tower with Pall Link supervisory control and data acquisition (SCADA) software package. The Allegro XRS 25 bioreactor system incorporates many features to ensure easy operation, robust performance, and suitability for cGMP operations.
Allegro XRS 25 Biocontainer
|3 dimensional biocontainer design||Superior mixing (with bi-axial platform),robust in operation|
|Biocontainer with integrated optical sensors, integrated filters, pre-assembled tubing sets||True single-use system, ease of installation, minimal turn-around time|
|Gas inlet and exhaust filters are integrity testable, sterilizing grade 0.2 μm||Improved suitability for cGMP operations|
|Integrated bottom drain with rotary lift valve||Improved suitability for cGMP operations, minimal hold up volume, ease of use|
|Multiple liquid addition ports||Improved ease of use, easily handles fed batch operations, acid/base pH control feeds|
|Integrated single use pH and dissolved oxygen (DO) sensors||Ease of use and reduced probability of contamination|
|Integrated sampling port||Ease of use - Very low hold up volume (less than 5 mL), capable of sampling without stopping agitation|
|Single-use biocontainer built with Allegro film||Assurance of robustness, quality and supply, track record of use of film in cGMP operations, validation guide available|
Allegro XRS 25 Platform
|Bi-axial agitation (rocking motion on two horizontal axes at 90 ° separation)||Improved agitation properties: shorter mixing times and higher mass transfer rates|
|Fully enclosed platform with safety interlock||Improved operator safety, suitable for production environments, protects light sensitive culture media|
mPath Control Tower and Pall Link Server
|mPath control tower designed for operation with Pall Link – a SCADA software package||
|Multiple alarm notification modalities to ensure response to process critical events||
|Gas handling by six oxygen-rated thermal mass flow controllers (TMFCs) pre-calibrated for process gases||
|Liquid handling with three variable speed pumps, integrated load cells and biocontainer hangers||
|Ergonomic industrial design||
|Tubing management system||In-process sampling||Drain and optical sensors|
Pall has standard Allegro XRS 25 biocontainers equipped with all liquid and gas connections for the most typical processes and most operations. The three feed lines, the accessory line, and the drain are fitted with standard laboratory connections. Process liquids, including media and inoculum, can easily and safely be transferred into the bioreactor.
Pall can assist in customizing the bioreactor systems. This includes adding aseptic connectors (Kleenpak® sterile connectors), and small changes on the biocontainer up to and including integrating the bioreactor with all surrounding unit operations into a fully automated process.
Documentation and Certificates
The Allegro XRS bioreactor is delivered with complete documentation that qualifies the system. All units are tested prior to shipment and witnessed Factory Acceptance Testing (FAT) is available on request.
The biocontainers are delivered with all the batch specific certificates and calibration information for the included single-use sensors.
Pall also can assist with hardware qualification, and standard installation verification (IV), operational verification (OV), factory acceptance test (FAT) and site acceptance test (SAT) test protocols are available.
The mixing properties of the 3D biocontainer design and bi-axial agitation lead to superior cell culture performance in mammalian suspension systems. With faster delivery of nutrients to the cellular micro-environment (and faster removal of metabolites), the Allegro XRS 25 bioreactor system allows cells to attain higher densities, with higher overall viabilities, leading to significantly higher protein expression levels. In the batch culture example below (Figure 1), with identical media inputs and seeds, the Allegro XRS 25 bioreactor system produced 18% higher cell densities, and 30% more antibody titer compared to conventional rocker technologies.
Summary of side-by-side growth and viability experiments (n=3) at 20 liter working volumes comparing conventional rocker system and Allegro XRS 25 bioreactor system. Simple batch culture of a CHO (Chinese hamster ovary) cell line expressing a monoclonal antibody
The excellent mixing properties of the Allegro XRS 25 bioreactor system have also shown to be beneficial for growing batch, fed batch and perfusion cultures of mammalian (CHO, hybridoma) and insect cells.
General System Specifications
(W x D x H)
|600 mm x 450 mm x 230 mm
664 mm x 379 mm x 331 mm (allow for 200 mm lid overhang at the rear of the platform, and factor in an additional 370 mm for height when the lid is open)
Platform 78 kg (104 kg filled) (with filled biocontainer)
78 kg (104 kg filled)
Gas flow control
TMFC pressurized air
TMFC carbon dioxide
|Inlet and outlet quick connectors 6 mm OD
0 - 1000 mL/min
0 - 1000 mL/min
0 - 1000 mL/min
0 - 1000 mL/min
|Pumps||Quantity and connection||3 x variable speed peristaltic pumps, 0 - 211 rpm, maximum tubing OD 8 mm|
|Aeration||Inlet filter||0.2 μm single-use filter|
|Gas mix injected into
0.2 μm single-use filter
Silicone heater mat
|Bi-Axial Agitation||Platinum RTD probeSpeed||1 - 35
|-15 to +15 degrees in both X and Y directions
Stepper motor controlled
Temperature control method
Dissolved oxygen control
|2 - 25 liters
Heating blanket, ambient convective cooling
10 - 40 °C
6 - 8, integrated single-use optical sensor
0 - 100%, integrated single-use optical sensor
The Allegro XRS 25 bioreactor system is available as a standard product; however, both the bioreactor hardware and consumables can be customized. Our teams are specialized in upstream and downstream processing and will gladly help find the right technology for any part of your manufacturing process. It is possible to combine the Allegro products to meet any requirement in the upstream part of the process:
- Media preparation
- Buffer preparation
- Media sterilization and aseptic transfer
- Liquid transfer into the bioreactor (e.g. glucose, anti-foam, base, etc.)
- Seed train solutions
- Cell harvest and separation
- Perfusion modules
Please contact Pall for a total solution discussion on your process.
Allegro XRS 25 bioreactor system integrated into
Pall Quality Standards
Pall maintains a very stringent approach to quality of purchased and manufactured components. Allegro bioreactor systems are designed and built to
- Good automated manufacturing practice (GAMP) current version
- 21 CFR Part 11 for electronic records
- Electromagnetic compatibility (EMC) to EN 61326-1: 2013 and FCC CFR 47 Part 15B: 2013
- The product is made in a manufacturing facility where the quality management system is ISO9001: 2008 certified.
A full list of quality standards is available on request.
The Allegro XRS 25
- The product is made in a manufacturing facility where the quality management system is ISO13485 and ISO9001 certified
- 100% of Allegro XRS 25
biocontainersare checked for correct components, configuration, welding standard and dimensional correctness. A visual inspection confirms there are no embedded particles in the film or any loose particles inside or outside of the biocontainer
- 100% of Allegro XRS 25
biocontainerchambers undergo a pressure leak test during manufacturing
- Samples from each
biocontainermanufacturing batch undergo a tensile test of the film weld area and a microscope inspection of the weld area to ensure the quality of the welded seal
- Fluid path endotoxins: periodically, rinse effluents from representative samples of Allegro systems are tested for endotoxins in accordance with USP <85> using
limulusamoebocyte lysate (LAL) reagent. Fluid path rinses meet the internal specification of < 0.25 EU/mL
- Fluid path cleanliness: periodically, rinse effluents from representative samples of Allegro systems are tested for particulates. Fluid path rinses meet the current limits under USP <788> particulate matter in injections
The materials of construction of the Allegro XRS 25
- USP<88> Biological reactivity test in vivo for class VI - 50 °C plastics
- USP<87> Biological reactivity tests in vivo, cytotoxicity
- USP<85> Bacterial endotoxin tests
- USP<661> Physico-chemical testing for plastics
- USP<788> Particulate matter in injections
Materials of construction for all components in contact with fluid are TSE/BSE risk materials free.
All Allegro XRS 25
Preventive Maintenance and Service Packages
The Allegro single-use bioreactor systems are supported with extensive maintenance and service packages. Pall has a global network of technicians and engineers available to assist with installation, qualification, training, technical support, preventive maintenance and on-site repairs. Contact Pall to arrange to discuss any of these services.
Pall Accelerator process development services provide customers with expert advice and support for the implementation of fully integrated single-use processing solutions into new or existing processes, including process optimization, equipment selection, transfer of existing processes, scale-up and troubleshooting advice. Details can be found in the Accelerator Process Development Services brochure (USD 3079).
Preventive maintenance packages are available to ensure the
The Allegro XRS 25 bioreactor system replaces the XRS 20 bioreactor system. Pall will continue to support the XRS 20 bioreactor system with repair services and spares. Please contact your local Pall representative for more information.
|Allegro XRS 25 Bioreactor System:
System hardware – consists of Allegro XRS 25 platform, mPath controller, control cables
Pall Link server and SCADA software
Standard Allegro XRS 25 biocontainer
|Kleenpak™ manifold with vent filter
Luer to Y connection and Kleenpak connectors
MPC to Y connection and Kleenpak connectors
5 liter 2D Allegro accessory biocontainer with filling filter, Kleenpak connector
1 liter 2D Allegro accessory biocontainer with filling filter, Kleenpak connector
20 liter 2D Allegro accessory biocontainer with filling filter, Kleenpak connector